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Purpose: Provides Sample Temperature Stabilization Guidelines for Color Measurement

Liquids need to be stabilized before measurement. The stabilization time for liquids in transmittance spectrophotometry depends on several factors, including volume, thermal conductivity, heat capacity, and environmental conditions. Below is a structured way to determine and express the stabilization time. See the table below for guidelines.
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Liquid Type

Small Volume (<10 mL, 1-10 mm path)

Medium Volume (10-100 mL, 10-50 mm path)

Large Volume (>100 mL, bulk)

Water & Aqueous Solutions

10-15 min

20-30 min

45-60 min

Alcohols & Glycols

10 min

20 min

40-50 min

Hydrocarbons

5-10 min

15-25 min

30-45 min

Edible Oils & Fats

10-15 min

25-35 min

45-60 min

Solvents

5-10 min

15-20 min

30-45 min

Biological Fluids

15 min

30 min

60 min

Petroleum & Fuels

5-10 min

15-25 min

30-45 min

Note for exact stabilization times, refer to your corporate SOP.

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Keywords: Temperature, Sample Preparation